Journal: Arthritis Research & Therapy

Article Title: Identification of definitive serum biomarkers associated with disease activity in primary Sjögren’s syndrome

doi: 10.1186/s13075-016-1006-1

Figure Lengend Snippet: Correlation between European League Against Rheumatism Sjögren’s Syndrome Disease Activity Index ( ESSDAI ) scores and concentrations of nine screened serum proteins analyzed by ELISA in the validation cohort of patients with primary Sjögren’s syndrome ( n = 58). The nine proteins were CXCL13, TNF receptor 2 ( TNF-R2 ), CD48, B-cell activating factor ( BAFF ), programmed cell death protein 1 ligand 2 ( PD-L2 ), lymphocyte activation gene-3 ( LAG-3 ), β2-microglobulin ( β2MG ), ephrin type-B receptor 2 ( EPHB2 ), and CD163. Pearson’s correlation test was used. The correlation coefficients ( r ) and P values are shown in the table. P <0.05 was considered significant

Article Snippet: The following commercially available kits for ELISA were used: Human Ephrin Type-B Receptor 2 (EPHB2) ELISA Kit (CUSABIO, Wuhan, Hubei Province P.R., China), Quantikine® Human CXCL13/BLC/BCA-1 Immunoassay ELISA kit (R&D Systems, Minneapolis, MN, USA), Human CD48 ELISA Pair Set (Sino Biological Inc., Beijing P.R., China), β-2 Microgloblin Human SimpleStep ELISA TM Kit, (Abcam, Tokyo, Japan), Soluble TNF Receptor II Human ELISA Kit (Abcam), RayBio® Human LAG3 ELISA Kit (RayBiotech, Inc., Norcross, GA, USA), DuoSet® ELISA Human CD163 and DuoSet® Ancillary Reagent Kit2 (R&D Systems), and ELISA Kit for Programmed Cell Death Protein 1 Ligand 2 (PDCD1LG2) (Cloud-Clone Corp., Houston, TX, USA).

Techniques: Activity Assay, Enzyme-linked Immunosorbent Assay, Biomarker Discovery, Activation Assay